BRD4 (BD1+BD2)

Catalog #: 31047
Size: 100 µg
$295
Species: Human
Host Species: E. coli
MW: 46.9 kDa
Genbank #: NM_058243
Tag(s): none
a.a: 49–460
Description:
Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-460 corresponding to bromodomains 1 and 2, with no tag, MW = 46.9 kDa, expressed in an E. coli cell expression system.
UniProt
O60885
Synonym(s):
BRD4, Bromodomain, HUNK1, MCAP
Assay Conditions:
BET Ligand (Cat. #33000) was incubated with un-tagged BRD4 for 15 min at room temperature in 1X BRD assay buffer. GST-tagged BRD4 (Cat. #31044) was added and incubated for 20 minutes followed by addition of 20 ?l mixture of GSH Acceptor beads and Streptavidin-Donor beads for AlphaScreen® analysis. Total reaction mixture was incubated for 35 minutes and the AlphaScreen® signal was measured by EnSpire® Multilabel Plate Reader (PerkinElmer).
Formulation:
40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

Store at -80°C. Stable for >6 months when stored as directed.

Application(s):
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Reference(s):
1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May; 90(5): 587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov 29;12(11):133.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Bromodomain
Product Type:
Recombinant Protein
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Product Datasheets

31047_Lot130815-M.pdf