BRD4 (BD1+BD2), GST-tag

Catalog #: 31044
Size: 100 µg
$295
Species: Human
Host Species: E. coli
MW: 73.3 kDa
Genbank #: NM_058243
Tag(s): N-terminal GST-tag
a.a: 49-460
Description:
Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-460 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 73.3 kDa, expressed in an E. coli expression system.
UniProt
O60885
Synonym(s):
Bromodomain containing 4, BRD4, HUNK1, MCAP
Assay Conditions:

BRD4 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® ® GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen®® Streptavidin -conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Formulation:
40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Reference(s):
Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Recombinant Protein
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Product Datasheets

31044_Lot140529-4.pdf