BRD3 (BD1+BD2) TR-FRET Assay Kit
The BRD3 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD3 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD3, ligand, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
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Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips.
Catalog Number | Component | Amount | Storage | |
31035 | BRD3 (BD1+BD2) | 10 µg | -80°C | Avoid freeze/ thaw cycles! |
BET Bromodomain Ligand | 50 µl | -80°C | ||
Non-acetylated Ligand 1 | 15 µl | -80°C | ||
Tb donor | 2 x 10 µl | -20°C | ||
Dye-labeled acceptor | 2 x 10 µl | -20°C | ||
33012 | 3x BRD TR-FRET Assay Buffer 1 | 4 ml | -20°C | |
White, Nonbinding, low volume, microtiter plate | 1 | Room Temp. |
Filippakopoulos, P., et al., Cell 2012; 149:214.