BRD3 (BD1+BD2) TR-FRET Assay Kit

Catalog #
32615
$535 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The BRD3 (BD1+BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD3 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD3, ligand, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against BRD3 (BD1+BD2)? Check out our Bromodomain Screening Services.​

Synonyms
BRD3, RING3L, BRD-3
Product Info
Storage and Usage
Citations
Assay Kit Format
TR-FRET
Supplied As
Kit comes in a convenient format with purified BRD3 protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Materials Required But Not Supplied

Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips.

Format
Catalog Number Component Amount Storage 
31035 BRD3 (BD1+BD2) 10 µg -80°C



Avoid

freeze/
thaw
cycles!
  BET Bromodomain Ligand 50 µl -80°C
  Non-acetylated Ligand 1 15 µl -80°C
  Tb donor 2 x 10 µl -20°C
  Dye-labeled acceptor 2 x 10 µl -20°C
33012 3x BRD TR-FRET Assay Buffer 1 4 ml -20°C
  White, Nonbinding, low volume, microtiter plate 1 Room Temp.
UniProt #
Q15059
References

Filippakopoulos, P., et al., Cell 2012; 149:214.