BRD2 (BD2) Inhibitor Screening Assay Kit

Catalog #
32522
$510 *
Size: 384 reactions
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Description

The BRD2 (BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD2 bromodomain 2 (BD2) from binding to its substrate. The key to the BRD2 (BD2) Inhibitor Screening Assay Kit is the highly specific binding of the BRD2 bromodomain 2 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD2 bromodomain 2 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Synonyms
BRD2, RNF3, RING3, Bromodomain, activity assay kit, BRD-2
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified, GST-tagged BRD2 BD2 to perform a total of 384 enzyme reactions.
Materials Required But Not Supplied

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips

Format

COMPONENTS:

UniProt #
P25440
References
McBride, A.A., et al., Trends Microbiol. 2004; 12(12):527.