BRD1 TR-FRET Assay Kit
Catalog #
32625
$1,025
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Description
The BRD1 TR-FRET Assay Kit is designed to measure the inhibition of BRD1 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD1, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
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Synonyms
BRD-1
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
TR-FRET
Supplied As
Kit comes in a convenient format with purified BRD1 protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Materials Required But Not Supplied
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Format
COMPONENTS:
UniProt #
Q86X06
References
Filippakopoulos, P., et al., Cell 2012; 149:214.