BRAF (V600E) Kinase Assay Kit

Catalog #

48688

$560 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

B-raf, after stimulation by activated RAS, phosphorylates MEK1 resulting in activation of the mitogen-activated protein (MAP) kinase cascade. Since the MAPK cascade has been shown to be a critical factor in melanoma development, it has been proposed that targeting B-raf could be a promising approach to combat melanoma. More importantly, 40-60% of melanomas display a mutation in BRAF, e.g. B-Raf(V600E), which leads to constitutive activation of the MAP kinase pathway. The B-Raf(V600E) Kinase Assay Kit is designed to measure B-Raf(V600E) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. In addition, the B-Raf(V600E) Kinase Assay Kit includes wild type B-Raf enzyme as a positive control for researchers investigating B-Raf(V600E) specific inhibitors.

This product has been cited 11 times.

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Synonyms

BRAF1, RAFB1, B-raf 1, MGC126806, MGC138284, BRAF (V600E)

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Product Data Gallery

Product Info

Storage and Usage

Citations11

Assay Kit Format

Luminescent

Supplied As

The B-Raf(V600E) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant B-Raf enzyme, B-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Format

Catalog Number	Components	Amount	Storage
40533	B-Raf(V600E)	5 µg	-80°C	Avoid freeze/ thaw cycles!
40065	B-Raf(WT)*	1 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
79569	5X Raf substrate	1 ml	-80°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P15056

References

Cantwell-Dorris, ER, et al. Mol Cancer Ther. 2011 Mar;10(3):385-94

Obaid NM, et al. Int J Mol Sci. 2017 Mar 8;18(3). pii: E585.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinase

Regulatory Status

Research Use Only