BPTF/FALZ TR-FRET Assay Kit

Catalog #: 32632
Size: 384 reactions
$995
Description:
The BPTF TR-FRET Assay Kit is designed to measure the inhibition of the binding of BPTF, also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
UniProt
Q12830
Synonym(s):
BPTF, FALZ, HTRF
Supplied As:
Kit comes in a convenient format with purified BPTF/FALZ, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions
Format:

COMPONENTS:

Instructions for use:
See assay kit datasheet for detailed protocol.
Storage / Stability:
At least 6 months from date of receipt when stored as directed. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Filippakopoulos, P., et al., Cell 2012; 149:214.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Assay Kit
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Product Datasheets

32632.pdf