BMX, GST-tag Recombinant

Catalog #

40402

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant full-length human cytoplasmic tyrosine-protein kinase BMX (BMX), also known as bone marrow tyrosine kinase gene in chromosome X protein, epithelial and endothelial tyrosine kinase, ETK, and NTK38 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag, a.a. 1-675 (end). The gene accession number is NM_001721 (variant 2).

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Synonyms

ETK, PSCTK2, PSCTK3, bone marrow tyrosine kinase gene in chromosome X protein, epithelial and endothelial tyrosine kinase, NTK38

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

110 kDa

Amino Acids

full length

Specific Activity

43 pmol/min/µg

Genbank #

NM_001721

UniProt #

P51813

Tag(s)

N-terminal GST-tag

Background

The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells (1). The BMX gene is located on chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. BMX, is found to induce activation of the Stat signaling pathway (2).

References

1. Tamagnone, L. et al. Oncogene. 1994 Dec;9(12):3683-8.
2. Saharinen, P. et al. Blood. 1997 Dec 1;90(11):4341-53

Storage/Stability

>6 months at -80°C

Assay Conditions

Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Tyr,Glu) substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was measured using Kinase- Glo reagent (Promega)

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40402_Lot140709-5.pdf

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BMX, GST-tag Recombinant

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