ALK2, FLAG-Tag, Avi-Tag, Biotin-Labeled

Catalog #: 100176
Size: 10 µg
$370
Species: Human
Host Species: Sf9 insect cells
MW: 45 kDa
Genbank #: NM_1001105
Tag(s): N-terminal FLAG-Tag, C-terminal Avi-Tag
a.a: 147-509
Description:
Recombinant human ALK2 a.a. 147-509, (Genbank Accession No. NM_1001105) with an N-terminal FLAG-Tag and C-terminal Avi-Tag™, expressed in Sf9 cells using a baculovirus expression system. MW= 45 kDa.
UniProt
P32789
Synonym(s):
ALK2, Serine/threonine-protein kinase Haspin homolog ALK2
Assay Conditions:

Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ALK2—incubated 45 minutes at 30 °C. Followed by addition of 25 μL of BPS ADP-Glo reagent—incubated 45 minutes at room temperature. Followed by addition of 50 µl of BPS Kinase Detection Reagent—incubated 45 minutes at room temperature. Luminescence was measured.

Formulation:
Formulated in: 40mM Tris-HCl pH8.0, 110mM NaCl, 2.2mM KCl, 8ng/µL Flag peptide, 3mM DTT, and 20% glycerol.
Format:
Aqueous buffer solution.
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Kaplan, F., S., et al., Hum. Mutat. 2009;30:379-390.

2. Yu, P.B., et al., Nature Med. 2008;14:1363-1369.

Warning(s):

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category:
Kinase
Product Type:
Protein
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Product Datasheets

100176_Lot_180830-1.pdf