Mouse basic Fibroblast Growth Factor

Catalog #: 90112-B
Size: 50 µg
$200
Species: Mouse
Host Species: E. coli
MW: 16 kDa
Genbank #: P15655
a.a: 10–154
Background:
Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.
UniProt
P15655
Synonym(s):
bFGF, FGF2, Prostatropin, Basic Fibroblast Growth Factor, Fibroblast Growth Factor-2
Purity:
≥98% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Formulation:
Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Reference(s):

1. Przybylski M. J Wound Care. 2009 Dec,18(12):516-9.
2. Temple S, Qian X. Neuron. 1995 Aug,15(2):249-52.

Application Reference(s):
Pivotal Role of Phospholipase D1 in Tumor Necrosis Factor-α-Mediated Inflammation and Scar Formation after Myocardial Ischemia and Reperfusion in Mice (2014)

Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
P ALPEDGGAAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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