BCL2L2 TR-FRET Assay Kit

Catalog #

79587

$870 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2L2, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against BCL2L2? Check out our Apoptosis Screening Services.

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Synonyms

BCL2L2, BCL-W, Bcl-2-like protein 2, BCL2 like 2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Supplied As

The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format.

Materials Required But Not Supplied

Dithiothreitol (DTT, 0.5 M)
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format

Catalog Number	Component	Amount	Storage
100091	BCL2L2	10 µg	-80°C	*Avoid freeze/ thaw cycles!*
	BCL2 Peptide Ligand 2	400 Rxns	-80°C
30017	Anti-His Tb-labeled donor	2 x 10 μl	-20°C
	Dye-labeled acceptor	2 x 10 μl	-20°C
	3x MCL TR-FRET Assay Buffer*	4 ml	-20°C
	White, nonbinding, low volume, microtiter plate	1	Room Temp.

* Add 30 μl of 0.5M DTT before use.

UniProt #

Q92843

References

Kvansakul, M., Hinds, MG. Cell Death and Disease 2013; 4: e909

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Apoptosis

Regulatory Status

Research Use Only

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BCL2L2 TR-FRET Assay Kit

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