B7-H5 Fc Fusion, Avi-Tag, Biotin-Labeled (Human) Recombinant

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Catalog #

71327

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Description

Human secreted B7-H5 Fc fusion protein, also known as VISTA and VSIR, with C-terminal Avi-Tag. GenBank Accession No. NM_022153, a.a 33-194 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 47 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

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Synonyms

VISTA, VSIR

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Biotin-Avidin Pulldown

VSIG-3: VISTA [Biotinylated] inhibition curve using a chemiluminescent assay (#79782). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

B7-H5 (33-194-Fc(IgG1)-Avi)-(Biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

47 kDa + glycans

Amino Acids

33-194

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

NM_022153

UniProt #

Q9H7M9

Tag(s)

Fc Fusion, C-terminal Avi Tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

References

1. Zhu, Y., et al. Nature Communications. 2013 Feb 27; 4.

2. Jung, K., et al., Immune Network. 2013, Oct. 13;6:184-193.

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Datasheets/Protocols

71327 Lot 230301

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B7-H5 Fc Fusion, Avi-Tag, Biotin-Labeled (Human) Recombinant

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