B7-2 (CD86), Fc fusion, Biotin labeled (Human) HiP™ Recombinant

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Catalog #

71159

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Description

Human secreted B7-2, Fc fusion protein, also known as T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, and CD86. GenBank Accession No. NM_006889, a.a. 20-239 expressed in a HEK293 cell expression system. This protein is fused at the C-terminus to the Fc region of human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. MW = 53.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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Synonyms

T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, CD86, B72

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Gel Filtration Curve

CD28:B7-2[Biotinylated] inhibition curve using a chemiluminescent assay (#72062). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

53.8 kDa+glycans

Amino Acids

20-239

Genbank #

NM_006889

UniProt #

P42081

Tag(s)

C-terminal Avi-tag™

References

1. Ertelt, J.M., et al. J. Leukocyte Biol. 2013; 94(2): 367-376.
2. Kuchroo, V.K., et al., Cell. 1995; 80(5):707-718.

Storage/Stability

At least 6 months at -80°C

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and screening small molecules.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to 100 μg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71159_Lot150417.pdf

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B7-2 (CD86), Fc fusion, Biotin labeled (Human) HiP™ Recombinant

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