B7-1 (Woodchuck), Fc-fusion (Human), Avi-Tag, Biotin-labeled Recombinant

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Catalog #

100040

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Description

Woodchuck B7-1, also known as CD80, with C-terminal Avi-Tag fused to the Fc-region of Human IgG1. a.a 30-237, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 52 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

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Synonyms

CD80, with C-terminal Avi-Tag fused to the Fc-region of Human IgG1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Woodchuck CTLA4: B7-1 Interaction

Product Info

Storage and Usage

Citations

Species

Woodchuck

Host Species/Expression System

HEK293

Purity

≥90%

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

52kDa + glycans

Amino Acids

30-237

Tag(s)

C-terminal Avi-Tag

References

1. Byrum, J.N., Van Komen, J.S., Rodgers, W., J. Immunol. 2013 Sep 15;191(6):3073-3081.

2. Legat, A., et al., Front Immunol. 2013 Dec 19;4:455.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Reaction done similar to human CTLA4:B7-1[biotin] assay kit (BPS Catalog # 72009) protocol. Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only