Aurora A Protein Kinase, His-Tag (Sf9-derived) Recombinant

Catalog #

40004

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Aurora Kinase A also known as STK15, STK6, BTAK, HARK1, ARK1, and AURA, GenBank Accession No. NM_003600, a.a. 1-403 (end) with Nterminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=50 kDa.

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Synonyms

Aurora Kinase A, STK15, STK6, BTAK, HARK1, ARK1, AURA

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.

50 kDa

Amino Acids

1-403 (end)

Specific Activity

45 pmol/min/µg

Genbank #

NM_003600

UniProt #

O14965

Tag(s)

N-terminal His-tag

References

1. Ouchi,M. et al., J. Biol Chem. 279 (19), 19643-19648 (2004).
2. Kunitoku, N. et al., Dev. Cell 5 (6), 853-864 (2003).

Storage/Stability

>6 months at -80°C.

Assay Conditions

Assay was done in Kinase buffer containing 2 mM DTT using KEMPtide substrate (0.2 mg/ml) and 10 μM ATP. Reaction was done at 30oC for 45 min. Amount of ATP transferred was calculated using ADP-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40004_Lot_190402.pdf

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Aurora A Protein Kinase, His-Tag (Sf9-derived) Recombinant

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