Ataxin3 (MJD1, SCA3), His-tag Recombinant

Catalog #

80399

$290 *

Size: 250 µg

Qty

Bulk/Custom

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Description

Human Ataxin-3 (ATX3 or ATXN3), also known as Josephin (JOS), Machado-Joseph disease protein 1 (MJD1) or Spinocerebellar ataxia type 3 (SCA3), GenBank Accession No. NM_004993, a.a. 2-362(end) with an N-terminal His-tag, MW=42.1 kDa, expressed in E. coli.

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Synonyms

Ataxin-3, ATX3, ATXN3, Josephin, JOS, Machado-Joseph disease protein 1, MJD1, Spinocerebellar ataxia type 3, SCA3

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Ataxin-3 inhibition curve using a fluorogenic assay (#78044). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥60%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.

42 kDa

Amino Acids

2–362(end)

Genbank #

NM_004993

UniProt #

P54252

Tag(s)

N-terminal His-tag

References

1. do Carmo Costa, M., et al. (2010). PLoS One 5(7):e11728.
2. Jung, J., et al. (2009). Hum Mol Genet. 18(24):4843-52.
3. Macedo-Ribeiro, S., et al. (2009). PLoS One 4(6): e5834.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and Ataxin-3 (0-0.4 ng/µL) in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80399_Lot101019.pdf

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Ataxin3 (MJD1, SCA3), His-tag Recombinant

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