ATAD2B TR-FRET Assay Kit
The ATAD2B TR-FRET Assay Kit is designed to measure the inhibition of ATAD2B binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, ATAD2B, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against ATAD2B? Check out our Bromodomain Screening Services.
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Catalog # | Component | Amount | Storage | |
31125 | ATAD2B (KIAA1240), GST | 10 µg | -80°C |
Avoid Freeze/Thaw Cycles!! |
33000 | BET Bromodomain Ligand | 50 µl | -80°C | |
Non-acetylated Ligand 1 | 15 µl | -80°C | ||
Tb donor | 20 µl | -20°C | ||
Dye-labeled acceptor | 20 µl | -20°C | ||
33012 | 3x BRD TR-FRET Assay Buffer 1 | 4 ml | -20°C | |
79969 | White, Nonbinding Corning, low volume, microtiter plate | 1 | Room Temp |
Filippakopoulos, P., et al., Cell 2012; 149:214.