ATAD2B Inhibitor Screening Assay Kit

Catalog #
32605
$1,025 *
Size: 384 reactions
Qty
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Description

The ATAD2B Inhibitor Screening Assay Kit is designed to measure the inhibition of ATAD2B binding to its substrate. The key to the kit is the specific binding of the ATAD2B bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing ATAD2B and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Synonyms
activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Supplied As
The kit comes in a convenient AlphaLISA® format, with enough biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged ATAD2B bromodomain to perform a total of 384 enzyme reactions.
Materials Required But Not Supplied
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Format

COMPONENTS:

UniProt #
Q9ULI0
References
Filippakopoulos, P., et al., Cell 2012; 149:214.