The ATAD2A TR-FRET Assay Kit is designed to measure the inhibition of ATAD2A binding to its substrate in a homogeneous 384 reaction format. This FRETbased assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, ATAD2A, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against ATAD2A? Check out our Bromodomain Screening Services.
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Synonyms
KIAA1240, AAA domain containing 2B (variant 1)
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Product Data Gallery
Interaction of ATAD2A (BPS Bioscience # 31121) with peptide ligands
Kit comes in a convenient format with purified ATAD2A, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Materials Required But Not Supplied
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Applications
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
dry ice
Notes
The final concentration of DMSO should not exceed 0.5%.
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.