ARG1, His-Tag Recombinant

Catalog #

71658

$495 *

Size: 50 μg

Qty

Bulk/Custom

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Description

Human Arginase 1 (ARG1) protein, also known as LCN7 and LIECF3, GenBank Accession No. NM_000045, amino acids 1-322, with a C-terminal His-tag, expressed in an HEK293 cell expression system. MW = 36 kDa.

This product has been cited 3 times.

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Synonyms

arg1, arg2, arginase1, arginase2, arginine, arg, arg 1, arg 2, arginase 1, arginase 2, screening, arginase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

ARG1 inhibition curve using a colorimetric - UV Spectrophotometry assay (#72048). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

36 kDa

Amino Acids

1-322

Genbank #

NM_000045

UniProt #

P05089

Tag(s)

C terminal His-Tag

References

1. Sedbrook, J.C., et al., PNAS. 1999 Feb; 96(3):1140-1145.

2. Woll, P.J., et al., PNAS. 1998 Mar; 85(6):1859-1863.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Assay Conditions

The assay was performed by UV absorption using recombinant ARG1 with thioarginine substrate (Cayman 80240). The UV absorption signal at 412 nm is proportional to the amount of reaction product produced. Reactions were conducted for 30 min at room temperature. To initiate the reaction various concentrations of ARG1 (0-100 nM final concentration) were added to ARG1 assay buffer containing the thioarginine substrate. Absorption signals were measured using a Tecan Infinite M1000 plate reader.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only