ARG1 Inhibitor Screening Assay Kit

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Catalog #
72048
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Description

The ARG1 Inhibitor Screening Assay Kit is designed to measure ARG1 (arginase 1) activity for screening and profiling applications. The assay kit comes in a convenient 96-well or 384-well format, with enough purified recombinant ARG1 (amino acids 1-322), thioarginine substrate, assay buffer and detection reagent for 96 or 384 enzyme reactions.

Synonyms
arg1, arginase 2, arg2, arg 2, arginase1, arginase2, arginine, arg, arg 1, arg 2, arginase 1, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Colorimetric - UV Spectrophotometry
Materials Required But Not Supplied
  • Spectrophotometer capable of measuring absorbance at λ= 410–415 nm.
  • Ethanol (200 proof)
Format

96 reactions

Catalog # Name Amount Storage
71658 ARG1, His-Tag* 25 µg -80°C
  10x ARG Assay Buffer 1 ml -80°C
  Thioarginine 1 mg -80°C
  Detection Reagent 3 mg -80°C
79965 UV Transparent 96-well plate 1 Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

 

384 reactions

Catalog # Name Amount Storage
71658 ARG1, His-Tag* 50 µg -80°C
  10x ARG Assay Buffer 5 ml -80°C
  Thioarginine 3 x 1 mg -80°C
  Detection Reagent 3 mg -80°C
79962 UV Transparent 96-well plate 1 Room Temp

* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

UniProt #
P05089
Background

Arginase enzymes convert arginine to ornithine through hydrolysis. Two known isoforms of Arginase exist, ARG1 and ARG2. This enzyme is involved in the regulation of a variety of immunological responses and is a major target in immunotherapy. ARG1/2 is overexpressed in myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs). Overexpression of ARG1/2 results in depleted levels of arginine both intracellularly and extracellularly. As arginine levels are depleted in the microenvironment, immune cells are starved of this amino acid and the function of key immunological activators become impaired; T cell proliferation is inhibited, regulatory T cells become activated and inhibit CD4+ T cells, and immunosuppressants have increased longevity. Depleted arginine also results in the release of reactive nitrogen species and reactive oxygen species from TAMs and MDSCs. These reactive species cause T cell apoptosis and the activation and growth of antigen presenting cells.

References

Sedbrook J.C., et al., 1999 PNAS 96(3):1140-1145.  
Woll P.J., et al., 1998 PNAS 85(6):1859-1863.