BCL2L2, His-Tag

Catalog #: 100091
Size: 100 µg
$405
Species: Human
Host Species: E. coli
MW: 20 kDa
Genbank #: NM_004050
Tag(s): C-terminal His-tag
a.a: 1-172
Description:
Human BCL2L2, also known as Bcl-2-like protein 2, and BCL2 like 2, a. a. 1-172, with C-terminal His-tag, GenBank Accession No. NM_004050, MW = 20 kDa, expressed in an E. coli expression system.
Synonym(s):
Bcl-2-like protein 2, BCL2 like 2
Assay Conditions:

Add 20-40 ng of BCL2L2 to 20 μl reaction mix containing 130 nM MCL1 substrate, anti-His Tb-labeled donor, and dyelabeled acceptor. Incubate 1h at room temperature. Read TR-FRET (λexc=340 nm, λem1=620 nm and λem2=665 nm).

Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3mM DTT and Imidazole
Storage / Stability:
At least 6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, selectivity profiling, and as a positive control for western blot studies.
Reference(s):

1. Shi, G. et al., Experimental Brain Research. Nov; 216(2) : 225-230 (2012)

2. Qu J. et al., Journal of Cellular Physiology.  230(3): 1868-1878 (2018)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Apoptosis
Product Type:
Protein
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Product Datasheets

100091.pdf