BCL-XL, His-tag

Catalog #: 50273
Size: 100 µg
$405
Species: Human
Host Species: E. coli
MW: 28 kDa
Genbank #: Z23115
Tag(s): C-terminal His-tag
a.a: 1–212
Background:
BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.
Description:
Human BCL-XL (GenBank Accession No. Z23115), a.a. 1-212 with C-terminal His-tag, MW = 28 kDa, expressed in an E. coli expression system.
UniProt
Q07817
Synonym(s):
Bcl-Xlong, B cell lymphoma XL, bclxl, bcl-2
Assay Conditions:
Following the protocol for BPS Cat. #50223. Briefly, BCL-XL was combined with BCL-XL peptide ligand in TR-FRET assay buffer along with anti-His Tb donor and dye-labeled acceptor. Varying concentrations of ABT 199 were added and the reaction was incubated at room temperature for 2 hours followed by fluorescent intensity measurements.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole and 3 mM DTT.
Format:
Aqueous buffer solution
Application(s):
Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
Reference(s):
1. Meng Y. et al., Mol Cancer Ther. 2008 Jul;7(7):2192-202.
2. Asakura T. et al., Mol Int J Oncol. 2008 Aug;33(2):389-95.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Apoptosis
Product Type:
Recombinant Protein
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50273_Lot_90116.pdf