BCL-2, His-tag

Catalog #: 50272
Size: 100 ยตg
$405
Species: Human
Host Species: E. coli
MW: 27 kDa
Genbank #: P10415
Tag(s): C-terminal His-tag
a.a: 1–211
Background:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Description:
Human Bcl-2 (GenBank Accession No. P10415), a.a. 1-211 with C-terminal His-tag, MW = 27 kDa, expressed in an E. coli expression system.
UniProt
P10415
Synonym(s):
Bcl, Bcl2, B cell lymphoma 2
Assay Conditions:
Following the protocol for BPS Cat. #50222. Briefly, BCL-2 was combined with BCL-2 peptide ligand in TR-FRET assay buffer along with anti-His Tb donor and dyelabeled acceptor. Varying concentrations of ABT 199 were added and the reaction was incubated at room temperature for 2 hours followed by fluorescent intensity measurements.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Format:
Aqueous buffer solution
Application(s):
Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
Reference(s):
1. Labi V. et al., Cell Death Differ. 2008 Jun;15(6):977-87.
2. Rong Y.P. et al., Mol Cell. 2008 Jul 25;31(2):255-65.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Apoptosis
Product Type:
Recombinant Protein
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50272_Lot140325-A.pdf