XIAP (Bir3), His-tag

Catalog #: 75001
Size: 50 µg
$310
Species: Human
Host Species: E. coli
MW: 14.2 kDa
Genbank #: NM_001167
Tag(s): N-terminal His-tag
a.a: 241–356
Description:
Human x-linked inhibitor of apoptosis (XIAP) Bir3 domain, also known as IAP-like protein, API3, X-linked IAP, and BIRC4, amino-acids 241 – 356 (GenBank Accession No. NM_001167) with N-terminal His-tag, MW = 14.2 kDa, expressed in an E. coli expression system.
UniProt
P98170
Synonym(s):
XIAP, IAP-like protein, API3, X-linked IAP, BIRC4
Assay Conditions:
50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at exc 475 nm, em 528 nm.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful in binding and inhibition studies of Caspase 9, screening inhibitors, and selectivity profiling.
Reference(s):
1. Nikolovska-Coleska, Z., et al., Anal Biochem. 2008 Mar 1;374(1):87-98.
2. Verhagen, A.M., et al., Cell Death Differ. 2007 Feb;14(2):348-57
3. Fulda, S., Vucic., D., Nat Rev Drug Discov. 2012 Feb 1;11(2):109-24
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Apoptosis
Product Type:
Recombinant Protein
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Product Datasheets

75001_Lot120718.pdf