BCL2L2 TR-FRET Assay Kit

Catalog #: 79587
Size: 384 reactions
$765
Description:
The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2L2, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Materials Required But Not Provided:
  • • Dithiothreitol (DTT, 0.5 M)
  • • Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
  • • Adjustable micropipettor and sterile tips
Synonym(s):
BCL2L2, BCL-W, Bcl-2-like protein 2, BCL2 like 2
Supplied As:
The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format.
Format:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Kvansakul, M., Hinds, MG. Cell Death and Disease 2013; 4: e909

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Apoptosis
Product Type:
Assay Kit
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Product Datasheets

79587.pdf