BCL-2 TR-FRET Assay Kit

Catalog #: 50222
Size: 384 reactions
$765
Description:
The BCL-2 TR-FRET Assay Kit is designed to measure the inhibition of BCL-2 binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbiumlabeled donor, dye-labeled acceptor, BCL-2, peptide ligand, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Synonym(s):
Bcl, Bcl2, B cell lymphoma 2
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
At least 6 months from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Filippakopoulos, P., et al.,Cell 2012; 149:214.
Notes:
Since January of 2018, this kit has been optimized for better performance. The previous version of the BCL-2 TR-FRET Assay Kit, Cat. #50222, can still be purchased upon special request.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Apoptosis
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50222.pdf