Apoptosis Activator 2

Catalog #: 27733-2
Size: 50 mg
MW: 306.1 Da
CAS Registry #: 79183-19-0
Formula: C15H9Cl2NO2
Target: Caspase
Apoptosis Activator 2 is a cell-permeable indoledione compound that activates caspases in a cytochrome c-dependent manner and induces apoptosis in tumor cells by promoting the oligomerization of Apaf-1 into the mature apoptosome. Its cytotoxicity is selective towards tumor cells (IC50 < 10 µM for Jurkat, Molt-4, and CCRF-CEM). Apoptosis Activator 2 shows little or no effect on non-tumor cells or cells that are deficient in caspase-3 or Apaf-1 (IC50 > 40 µM). Through the induction of apoptosis, Apoptosis Activator 2 notably induces caspase-3 activation, PARP cleavage and DNA fragmentation. Moreover, this induction of apoptosis only occurs in tumor cell lines (such as breast, lung, colon, and epidermal cancer cell lines).
Apoptosis Activator 2 is a small, cell permeable activator of caspase-3. Apoptosis Activator 2 induces apoptosis specifically in tumor cell lines, but not normal cell lines, with IC50 value of about 4 µM.
Apoptosis Activator II; AG-H-17727; ZINC02276110; 1-[(3,4-dichlorophenyl)methyl]indole-2,3-dione
Biological Activity:
Induces apoptosis of tumor cell lines, including BT-549: IC50 = 20 µM; NCI-H23: IC50 = 35 µM; Jurkat: IC50 = 4 µM; Molt-4: IC50 = 6 µM; and CCRF-CEM: IC50 = 9 µM.
Soluble in DMSO (5 mg/ml), methanol (5 mg/ml), and ethanol.
Storage / Stability:

Store at or below –20°C. Solid form is stable at least 12 months from date of receipt, when stored as directed. Do not store aqueous solutions for more than one day.

1. Nguyen JT, Wells JA. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8.
2. Nguyen TV, et al. J Neuroendocrinol. 2010 Sep;22(9):1013-22.
Scientific Category:
Apoptosis Activator
Product Type:
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