Anti-V5 Tag
Mouse Monoclonal Antibody

Catalog #: 25001

Size: 100 μg

Clonality | Host Species | Isotype:
E10/V4RR | Mouse | IgG1

Formulation:
1mg/mL in 10mM PBS, pH 7.2, with 0.05%
NaN3

Sensitivity:
WB (with ECL): 1-2ng of V5-tagged fusion
proteins

Working Conditions:
WB (with ECL): 1:1000-3000 dilution
(incubate for 1 hour at room temperature)
ICC: 1:500-2000 dilution
For best results with other assays (e.g.: Dot
Blot, ELISA, IP, etc.), please determine
optimal working dilution by titration test

Purification:
Protein A affinity chromatography from
culture supernatant of hybridoma cells
grown in a bioreactor

Storage and Stability:
Store at -20°C for up to 2 years. Centrifuge
after first thaw to maximize product
recovery. Aliquot to avoid repeated freezethaw
cycles. Store aliquots at 4°C for 1-2
weeks

Specificity:
Recognizes V5 (GKPIPNPLLGLDST)
tagged fusion proteins

Immunogen:
GKPIPNPLLGLDST (V5) synthetic
peptide conjugated to KLH

Applications:
Dot Blot, ELISA, IP, ICC, WB

Click here to download the product data sheet.