Anti–G9a polyclonal antibody

Catalog #: 25224
Size: 100 µl
$280
Host Species: Rabbit
Clonality: polyclonal
Background:
G9a (UniProtKB/Swiss–Prot entry Q96KQ7) is a member of the Set 1 family of histone methyltransferases. It preferentially methylates K9 and K27 of histone H3. Methylation of H3K9 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9a is a part of the E2F6.com–1 complex.
Description:
Polyclonal antibody raised in rabbit against mouse G9a (Protein G9a), using two KLH–conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.
Synonym(s):
EHMT2, BAT8, KMT1C, NG36
Assay Conditions:

Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a (Cat. No. 25224). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.

Western blot analysis using the antibody directed against G9a
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a (Cat. No. 25224), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.

Concentration:
100 µl
Formulation:
Whole antiserum from rabbit containing 0.05% azide
Species Reactivity:
Mouse
Purification:
Whole serum
Immunogen:
synthetic peptide
Format:
aqueous solution
Storage / Stability:

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Application(s):
ELISA (1:500 – 1:1000)
WB (1:1000)
Notes:
The optimal antibody concentration should be determined by the end–user.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Antibodies
Product Type:
Antibody
Data shown is lot-specific. Contact us for specific information on other lots.

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