Anti–EzH2 polyclonal antibody

Catalog #: 25223
Size: 50 µg
$365
Host Species: Rabbit
Clonality: polyclonal
Background:
EZH2 (UniProt/Swiss–Prot entry Q15910) is a histone–lysine methyltransferase which methylates Lys–9 and Lys–27 of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and central nervous systems. Over–expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer.
Description:
Polyclonal antibody raised in rabbit against the N–terminus (aa1–343) of the mouse EZH2 protein (Enhancer of zeste homolog 2).
Synonym(s):
ENX–1, KMT6
Assay Conditions:

ChIP results obtained with the antibody directed against EZH2
ChIP assays were performed using HeLa cells, the antibody against EZH2 (Cat. # 25223) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 0.5, 1, 2.5 and 5 µg per ChIP experiment was analyzed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1, used as a positive control target, and for the promoter of the active GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western blot analysis using the antibody directed against EZH2
Nuclear extracts of HeLa cells (40 µg) were analyzed by Western blot using the antibody against EZH2 (Cat. # 25223) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size 85 kDa) is indicated on the right; the marker (in kDa) is shown on the left. 

Concentration:
50 µg/50 µl
Formulation:
PBS containing 0.05% azide and 0.05% ProClin 300
Species Reactivity:
Human, mouse
Purification:
Protein G purified
Immunogen:
recombinant protein
Format:
Aqueous buffer solution
Storage / Stability:

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Application(s):
ChIP (2.5 µg/ChIP)
WB (1:1000)
Immunochemistry (1:100 – 1:500)
Notes:
The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Antibodies
Product Type:
Antibody
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