Anti–EZH2 monoclonal antibody

Catalog #: 25222
Size: 50 µg
$365
Host Species: Mouse
Clonality: monoclonal
Isotype: IgG1
Background:
EZH2 (UniProt/Swiss–Prot entry Q15910) is a histone–lysine methyltransferase which methylates Lys–9 and Lys–27 of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and central nervous systems. Over–expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer.
Description:
Monoclonal antibody raised in mouse against the central part of the human EZH2 protein (Enhancer of zeste homolog 2)
Synonym(s):
ENX–1, KMT6
Assay Conditions:

ChIP results obtained with the monoclonal antibody directed against EZH2
ChIP assays were performed using 2.5 µg of the monoclonal antibody against EZH2 (Cat. # 25222) on sheared chromatin from 5 million E14 mouse embryonic stem and from SUZ12-/- cells, used as a negative control. IgG was used as negative IP control. Quantitative PCR was performed with primers specific for the HoxA10 gene and for the T gene which encodes the Brachyury transcription factor. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). 

Concentration:
50 µg/50 µl
Formulation:
PBS containing 0.05% azide
Species Reactivity:
Human, mouse
Purification:
Protein G purified
Immunogen:
recombinant protein
Format:
Aqueous buffer solution
Storage / Stability:

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Application(s):
ChIP (2.5 µg/ChIP)
Notes:
The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Antibodies
Product Type:
Antibody
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