Anti–ER alpha monoclonal antibody

Catalog #: 25221
Size: 100 µl
$365
Host Species: Mouse
Clonality: monoclonal
Background:
The estrogen receptor alpha (ERalpha, UniProt/Swiss–Prot entry P03372) belongs to the family of nuclear hormone receptors, which are ligand–activated transcription factors. They are important for the regulation of gene expression, cellular proliferation and differentiation, sexual development and reproductive function. Estrogen receptors are also involved in pathological processes such as breast cancer, and osteoporosis. ERalpha can regulate transcription by direct binding to estrogen response elements (EREs) in the DNA or by interaction with other transcription factors. It may also form a heterodimer with ERbeta.
Description:
Monoclonal antibody raised in mouse against human ERalpha (estrogen receptor alpha), using a synthetic peptide
Synonym(s):
ESR, ESR1, ESRA, NR3A14
Assay Conditions:

ChIP-seq results obtained with the monoclonal antibody directed against ERalpha
ChIP was performed with the monoclonal antibody against ERalpha (Cat. # 25221) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The immunoprecipitated DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).

Concentration:
100 µl
Formulation:
Ascites fluid from mouse containing 0.05% azide
Species Reactivity:
Human
Cross Reactivity:
Does not react with chicken; other species not tested
Purification:
Ascites fluid
Immunogen:
synthetic peptide
Format:
aqueous solution
Storage / Stability:

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Application(s):
ChIP/ChIP – seq (2.5 µl)
ELISA (1:500 – 1:5000)
WB (1:500 – 1:5000)
Gel Supershift (1:10 – 1:20)
Immunochemistry (1:500 – 1:5000)
IP (1:200 – 1:5000 )
Notes:
The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Antibodies
Product Type:
Antibody
Data shown is lot-specific. Contact us for specific information on other lots.

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