Anti–DNMT3A polyclonal antibody

Catalog #: 25218
Size: 50 µg
$365
Host Species: Rabbit
Clonality: polyclonal
Background:
DNMT3A (UniProtKB/Swiss–Prot entry Q9Y6K1) catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.
Description:
Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH–conjugated synthetic peptide corresponding to amino acids 92–106.
Assay Conditions:

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human DNMT3A (Cat. # 25218), crude serum and flow-through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.

Western blot and Immunoprecipitation using the antibody directed against DNMT3A
Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 µg of antibody against DNMT3A (Cat. # 25218). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by Western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and Western blot analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3).

Concentration:
50 µg/54 µl
Formulation:
PBS containing 0.05% azide and 0.05% ProClin 300
Species Reactivity:
Human
Purification:
Affinity purified
Immunogen:
synthetic peptide
Format:
Aqueous buffer solution
Storage / Stability:

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Application(s):
ELISA (1:100)
WB (1:2
500)
IP (2 µg/IP)
Notes:
The optimal antibody concentration should be determined by the end–user.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Antibodies
Product Type:
Antibody
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance