Anti–Chd1 polyclonal antibody

Catalog #: 25213
Size: 50 µg
$280
Host Species: Rabbit
Clonality: polyclonal
Background:
CHD1 (UniProtKB/Swiss–Prot entry O14646) is an ATP–dependent chromatin remodeling factor, containing two tandem chromodomains. In human both chromodomains are essential for specific binding to histone H3, methylated at lysine 4. CHD1 belongs to the SNF2/RAD54 helicase family and contains also one helicase ATP–binding domain and one helicase C–terminal domain. It may play an important role in gene regulation.
Description:
Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain helicase DNA binding protein 1), using a GST–fused recombinant protein.
Synonym(s):
CHD–1, ATPase DNA–binding protein 1
Assay Conditions:

Western blot analysis using the antibody directed against CHD1
Western blot was performed on nuclear extracts from HeLa cells (HeLa nuclear extracts, 20 μg) with the antibody against CHD1 (Cat. No. 25213) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

Concentration:
50 µg/25 µl
Formulation:
PBS containing 0.05% azide and 0.05% ProClin 300.
Species Reactivity:
Human
Purification:
Affinity purified
Immunogen:
recombinant protein
Format:
Aqueous buffer solution
Storage / Stability:

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Application(s):
WB (1:500)
Notes:
The optimal antibody concentration should be determined by the end–user.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Antibodies
Product Type:
Antibody
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