Anti–JARID2 polyclonal antibody

Catalog #

25295

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against human JARID2 (jumonji, AT rich interactive domain 2) using two KLH–conjugated synthetic peptides containing a sequence from the N–terminal and the central region of the protein, respectively.

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Synonyms

JMJ

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

JARID2 (UniProtKB/Swiss–Prot entry Q92833) is a regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. JARID2 acts by recruiting histone methyltransferase complexes (PRC2) to their target genes and modulating the histone methyltransferase activity. It does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of H3K27me3 by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin–D1 (CCND1) by activating H3K9 methylation by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human JARID2 (Cat. # 25295). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,300.

Applications

ELISA (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–JARID2 polyclonal antibody

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