Anti–Jarid1b polyclonal antibody

Catalog #

25293

$335 *

Size: 100 µl

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Polyclonal antibody raised in rabbit against mouse Jarid1b (Jumonji, AT rich interactive domain 1B), using a KLH–conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

●

Synonyms

KDM5B, CT31, PLU1, PUT1, RBBP2H1, RBP2–like

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

JARID1B (UniProt/Swiss–Prot entry Q9UGL1) is a histone demethylase which specifically demethylates mono–, di, and trimethylated lysine 4 of histone H3. It acts as a transcriptional repressor for the tumor suppressor genes BRCA1 and HOXA5 and has been associated with breast cancer development. In contrast, in melanoma JARID1B itself may function as a tumor suppressor.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Jarid1b (Cat. # 25293). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,200.

Western blot analysis using the antibody directed against Jarid1b
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Jarid1b (Cat. # 25293), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.

Applications

ELISA (1:100 – 1:200)
WB (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Anti–Jarid1b polyclonal antibody

PRODUCTS

SERVICES

RESOURCES

COMPANY