Anti–H3K27ac monoclonal antibody

Catalog #

25238

$415 *

Size: 50 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Monoclonal antibody raised in mouse against histone H3 acetylated at lysine 27 (H3K27ac), using a KLH–conjugated synthetic peptide

This product has been cited 1 time.

●

Product Data Gallery

Product Info

Storage and Usage

Citations1

Isotype

IgG1

Clonality

monoclonal

Host Species/Expression System

Mouse

Purification

Protein A purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide

Concentration

1 µg/µl

Immunogen

synthetic peptide

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Cross reactivity of the monoclonal antibody directed against H3K27ac
To test the specificity an ELISA was performed using a serial dilution of the monoclonal antibody against H3K27ac (Cat. No. 25238). The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure 1 shows a high specificity of the antibody for the peptide containing the modification of interest.

Immunofluorescence using the monoclonal antibody directed against H3K27ac
HeLa cells were stained with the antibody against H3K27ac (Cat. No. 25238) and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Applications

ChIP (1 - 2 µg per IP)
ELISA (1:3000)
WB (1:1000 – 1:2000)
IF (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

25238 Lot 220324

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

Anti–H3K27ac monoclonal antibody

PRODUCTS

SERVICES

RESOURCES

COMPANY