Anti–EzH2 polyclonal antibody

Catalog #

25223

$415 *

Size: 50 µg

Qty

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Description

Polyclonal antibody raised in rabbit against the N–terminus (aa1–343) of the mouse EZH2 protein (Enhancer of zeste homolog 2).

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Synonyms

ENX–1, KMT6

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Protein G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300

Concentration

50 µg/50 µl

Immunogen

recombinant protein

Background

EZH2 (UniProt/Swiss–Prot entry Q15910) is a histone–lysine methyltransferase which methylates Lys–9 and Lys–27 of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and central nervous systems. Over–expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

ChIP results obtained with the antibody directed against EZH2
ChIP assays were performed using HeLa cells, the antibody against EZH2 (Cat. # 25223) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 0.5, 1, 2.5 and 5 µg per ChIP experiment was analyzed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1, used as a positive control target, and for the promoter of the active GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western blot analysis using the antibody directed against EZH2
Nuclear extracts of HeLa cells (40 µg) were analyzed by Western blot using the antibody against EZH2 (Cat. # 25223) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size 85 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

Applications

ChIP (2.5 µg/ChIP)
WB (1:1000)
Immunochemistry (1:100 – 1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human, mouse

Notes

The optimal antibody amount per IP should be determined by the end–user. We recommend testing 1–5 µg per IP

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–EzH2 polyclonal antibody

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