Anti–DNMT2 polyclonal antibody

Catalog #

25217

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against mouse DNMT2 (DNA methyltransferase 2), using a KLHconjugated synthetic peptide containing an amino acid sequence from the N–terminal part of the protein

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Synonyms

TRDMT1, M.HsaIIP, PuMet, RNMT1, DNMT-2

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

DNMT2 (UniProtKB/Swiss–Prot entry O14717) belongs to the family of DNA cytosine methyltransferases. DNMT2, however, lacks the large N–terminal regulatory domain common to other eukaryotic methyltransferases and does not methylate CpG in genomic DNA. Instead, it specifically methylates cytosine 38 in the anticodon loop of Asp transfer RNA.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse DNMT2 (Cat. No. 25217). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:21,000.

Western blot analysis using the antibody directed against DNMT2
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse DNMT2 (Cat. No. 25217), diluted 1:1000 in BSA/PBS-Tween-20. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (47 kDa) is indicated on the right.

Applications

ELISA (1:100 – 1:1000)
WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–DNMT2 polyclonal antibody

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