Anti-GAPDH, Monoclonal

Catalog #: 25007
Size: 100 µg
$325
Host Species: Mouse
Clonality: clone GA1R
Isotype: IgG1
Background:
Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Antibodies to GADPH are often used as "loading controls" because the enzyme is so ubiquitous.
Description:
Affinity purified Anti-GAPDH clone GA1R, mouse IgG1. Recognizes native and denatured forms of GAPDH (~37 kDa). Applications: Western Blotting(1:1000-10000), Dot Blot, ELISA, Immunocytochemistry, Immunofluorescence assays. Optimal dilutions/concentrations should be determined by the end user.
Synonym(s):
Antibody, GAPDH-tag
Formulation:
1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Species Reactivity:
Reacts with GADPH (~37 kDa) from Human, Mouse, Rat, Rabbit, Chicken, Hamster, Saccharomyces cerevisiae, Schizosaccharomyces pombe.
Cross Reactivity:
Predicted to work with a wide range of other species.
Purification:
Protein A Affinity Chromatography
Immunogen:
Recombinant GAPDH.
Format:
Aqueous buffer solution
Storage / Stability:
Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Application(s):
WB, DB, ELISA, ICC, IF
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Antibodies
Product Type:
Antibody
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

25007.pdf