Anti-CD3 Agonist Antibody

Catalog #: 71274-2
Size: 100 µg
$465
Host Species: HEK293
MW: Heavy Chain: 52 kDa, Light Chain: 26 kDa
Tag(s): none
Clonality: Monoclonal
Isotype: IgG1
Description:
Recombinant human antibody recognizing the CD3 binding region on immune cells.
Assay Conditions:
IL-2 luciferase Jurkat reporter cells were incubated with anti-CD3 (BPS Cat. No 71274) in SF-RPMI for 24 hours. BPS luciferase reagent was added and luminescence was quantified. RLU = Relative Luminescence Unit normalized to wells with RPMI only.
Formulation:
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Purification:
Protein A affinity chromatography from HEK293 supernatants.
Storage / Stability:
Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 – 0.5%) at 4°C, stable for 2 weeks.
Application(s):
Useful for the detection of CD3 and binding to immune cells expressing CD3 on their surface and useful as a functional grade antibody for T cell activation and expansion.
Reference(s):
1. Chatenoud, L., et al. PNAS. 1994; 91(1): 123-127.

2. Herold, K., et al., NEJM. 2002; 346: 1692-1698.

Scientific Category:
Immunotherapy
Product Type:
Antibody
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Product Datasheets

71274-2.pdf