AMPK (A2/B2/G2), His-tag Recombinant

Catalog #
40706
$510 *
Size: 5 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Recombinant human AMPK (combination of A2/B2/G2 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 69 kDa (A2), 36 kDa (B2), 65 kDa (G2).

Synonyms
Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA
Subunit B2: PRKAB2, MGC61468
Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity
≥75%
Format
Aqueous buffer solution
Formulation
50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
MW
~170 kDa (complex)
Amino Acids
full length
Genbank #
NM_006252 (A2), NM_005399 (B2), NM_001040633 (G2)
UniProt #
A2: P54646; B2: O43741; G2: Q9UGJ0
Tag(s)
C-terminal His-tag
Background
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
References
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.