AMPK (A2/B2/G1), His-tags Recombinant

Catalog #
40026
$690 *
Size: 10 µg
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Description

Recombinant human full-length AMPK (AMP-activated protein kinase), subunits A2/B2/G1. This construct contains a C-terminal His-tag. The recombinant protein was affinity purified and is active.

Synonyms
Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA
Subunit B2: PRKAB2, MGC61468
Subunit G1: PRKAG1, AMPKG, MGC8666
Product Info
Storage and Usage
Citations
Species
Human
Construct
AMPK (A2/B2/G1-His)
Host Species/Expression System
Sf9 insect cells
Purity

≥80%

Format
Aqueous buffer solution
Formulation

50 mM Sodium Phosphate, pH 7.0, 300 mM NaCl, 150 mM Imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

MW
A2: 69 kDa; B2: 36 kDa; G1: 41 kDa
Amino Acids
full length
Specific Activity

139 pmol/min/µg

Genbank #
NM_006252 (A2), NM_005399 (B2), NM_002733 (G1)
UniProt #
A2: P54646; B2: O43741; G1: P54619
Tag(s)
C-terminal His-tag
Background
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ (2).
References
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.