AMPK (A2/ B1/G1), His-tag Recombinant

Catalog #

40704

$510 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Recombinant human AMPK (combination of A2/B1/G1 subunits), full length, with C-terminal His-tag, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G1: PRKAG1, AMPKG, MGC8666

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

~148 kDa (complex)

Amino Acids

full length

Genbank #

NM_006252 (A2), NM_006253 (B1), NM_002733 (G1)

UniProt #

A2: P54646; B1: Q9Y478; G1: P54619

Tag(s)

C-terminal His-tag

Background

AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2).

References

1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Foretz, M. et al: Short-term overexpression of a constitutively active form of AMP-activated protein kinase in the liver leads to mild hypoglycemia and fatty liver. Diabetes, 2005; 54 (5);1331-1339.

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AMPK (A2/ B1/G1), His-tag Recombinant

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