AMPK (A1/B2/G1), His-tags Recombinant

Catalog #

40023

$690 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human AMPK (combination of A1/B2/G1 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 68 kDa (A1), 36 kDa (B2), 40 kDa (G1).

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Synonyms

Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B2: PRKAB2, MGC61468
Subunit G1: PRKAG1, AMPKG, MGC8666

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

~144 kDa (complex)

Amino Acids

full length

Genbank #

NM_006251 (A1), NM_005399 (B2), NM_002733 (G1)

UniProt #

A1: Q13131; B2: O43741; G1: P54619

Tag(s)

C-terminal His-tags

Background

AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The β subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle (2).

References

1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Thornton, C. et al: Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle. J. Biol. Chem. 273: 12443-12450, 1998.

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AMPK (A1/B2/G1), His-tags Recombinant

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