AMPK (A1/B1/G3), His-tag Recombinant

Catalog #
40702
$510 *
Size: 5 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Recombinant human AMPK (combination of A1/B1/G3 subunits) full length protein containing a C-terminal His-tag. This recombinant protein was affinity purified and is kinase active.

Synonyms
Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G3: PRKAG3
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity

≥70%

Format
Aqueous buffer solution
Formulation
50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
MW
A1: 68 kDa; B1: 38 kDa; G3: 51 kDa
Amino Acids
full length
Genbank #
NM_006251 (A1), NM_006253 (B1), NM_017431 (G3)
UniProt #
A1: Q13131; B1: Q9Y478; G3: Q9UGI9
Tag(s)
C-terminal His-tag
Background
AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The γ subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism (2).
References
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Cheung, P. C. F. et al: Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding. Biochem. J. 346: 659-669, 2000.