AMPK (A1/B1/G2), His-tag

Catalog #: 40701
Size: 5 µg
$470
Species: Human
Host Species: Sf9 insect cells
MW: ~171 kDa (complex)
Genbank #: NM_006251 (A1), NM_006253 (B1), NM_001040633 (G2)
Tag(s): C-terminal His-tag
a.a: full length
Background:
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
Description:
Recombinant human AMPK (combination of A1/B1/G2 subunits), full length, with C-terminal His-tags, expressed by Sf9 insect cells via a baculovirus expression system. MW = 68 kDa (A1), 38 kDa (B1), 65 kDa (G2).
Synonym(s):
Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p
Purity:
≥75%
Formulation:
50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
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