AMPK (A1/B1/G2), His-tags Recombinant

Catalog #
40021
$690 *
Size: 10 µg
Qty
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Description

Recombinant human AMPK (combination of A1/B1/G2 subunits) full length protein containing a C-terminal His-tag. This recombinant protein was affinity purified and is kinase active.

Synonyms
Subunit A1: PRKAA1, MGC33776, MGC57364
Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785
Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p
Product Info
Storage and Usage
Citations
Species
Human
Construct
AMPK (A1/B1/G2-His)
Host Species/Expression System
Sf9 insect cells
Purity

≥70%

Format
Aqueous buffer solution
Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. 

MW
A1: 68 kDa; B1: 38 kDa; G2: 65 kDa
Amino Acids
full length
Specific Activity

780 pmol/min/µg

Genbank #
NM_006251 (A1), NM_006253 (B1), NM_001040633 (G2)
UniProt #
A1: Q13131; B1: Q9Y478; G2: Q9UGJ0
Tag(s)
C-terminal His-tags
Background
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
References
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.