ALK2 (ACVR1), FLAG-Tag, Avi-Tag, Biotin-Labeled Recombinant

Catalog #

100176

$380 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human ALK2 a.a. 147-509, (Genbank Accession No. NM_001105.5) with an N-terminal FLAG-Tag and C-terminal Avi-Tag™, expressed in Sf9 cells using a baculovirus expression system. MW= 45 kDa.

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Synonyms

ALK2, Serine/threonine-protein kinase Haspin homolog ALK2

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Product Data Gallery

4-20% SDS-PAGE Coomassie staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

Formulated in: 40mM Tris-HCl pH8.0, 110mM NaCl, 2.2mM KCl, 8ng/µL Flag peptide, 3mM DTT, and 20% glycerol.

45 kDa

Amino Acids

147-509

Genbank #

NM_001105.5

UniProt #

Q04771

Tag(s)

N-terminal FLAG-Tag, C-terminal Avi-Tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

References

1. Kaplan, F., S., et al., Hum. Mutat. 2009;30:379-390.

2. Yu, P.B., et al., Nature Med. 2008;14:1363-1369.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ALK2—incubated 45 minutes at 30 °C. Followed by addition of 25 μL of BPS ADP-Glo reagent—incubated 45 minutes at room temperature. Followed by addition of 50 µl of BPS Kinase Detection Reagent—incubated 45 minutes at room temperature. Luminescence was measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Serine-Threonine Kinase

Regulatory Status

Research Use Only